Impact of Grape Leafroll Associated Virus-3 on Transcription Regulation and the Distribution of small RNA During Ripening
Amanda Vondras, Ph.D. student, Dr. Laurent Deluc, OSU Department of Horticulture
Grapevine leafroll associated virus 3 (GLRaV-3) is the most economically impactful virus affecting grapevine worldwide. However, there is little that can be done to reduce the consequences of viral infection for the plant on fruit production. Ultimately, targeted attempts to mitigate the effects of the virus will require an understanding of the plant-pathogen interaction at the molecular level, to understand how mechanisms that underpin normal fruit development are altered by the virus, and whether particular regulatory pathways might account for system-wide responses to the virus. Regulation of gene expression through small RNA and alternative splicing are essential components of plant development and responses to stress. We used Next Generation Sequencing technologies (RNA and small RNA sequencing) to assemble a holistic view of these regulatory agents during normal ripening in Vitis vinifera and how they are altered in response to GLRaV-3 infection.
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